PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.

136 – 150 of 3,795 results

136

Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix

AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.

Pricing & Availability
Specifications

Shipping Condition	Room Temp or Wet Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	1X
Overhang	3'-A
Concentration	2X
For Use With (Application)	Cloning,Hot-start PCR
Exonuclease Activity	5' - 3'
Polymerase	AmpliTaq Gold 360 DNA Polymerase
Format	Tube
Size (Final Product)	5 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	PCR Master Mix
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Starting Material	DNA

137

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase

The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript III
Final Product Type	PCR Amplified cDNA
PCR Method	1-step RT-PCR
Detection Method	Gel Electrophoresis
Polymerase	Platinum Taq High Fidelity
Format	One-Step RT-PCR System
Size (Final Product)	Up to 10 kb
Reaction Format	Premixed Components
Reaction Speed	30 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Hot Start	Built-In Hot Start
Optimal Reaction Temperature	50°C
Technique	1-Step RT-PCR
Starting Material	RNA

138

Applied Biosystems™ VetMAX™-Plus qPCR Master Mix

For use with VetMAX™ Reagents and VetMAX™ Controls.

Pricing & Availability

139

Invitrogen™ dNTP Mix (10 mM ea)

Invitrogen 10 mM dNTP Mix includes four nucleotides (dATP, dCTP, dGTP, dTTP) in 0.6 mM Tris-HCl. Each nucleotide is at a concentration of 10 mM and this mix is suitable for use in a wide variety of molecular processes.

Pricing & Availability
Specifications

Shipping Condition	Approved for shipment on Wet or Dry Ice
Form	Liquid
Label or Dye	Unlabeled
Concentration	10 mM
For Use With (Application)	Standard PCR,RT-PCR,Real Time PCR (qPCR)

140

Thermo Scientific™ MgCl₂ (magnesium chloride) (25 mM)

Thermo Scientific PCR reagents meet high quality standards to generate accurate results from even the most challenging molecular biology applications.

Pricing & Availability

141

Applied Biosystems™ SYBR™ Select Master Mix

Cost-effective solution for real-time PCR applications.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Content And Storage	The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.
Purity or Quality Grade	UP (Ultra Pure)
Form	Liquid
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Detection Method	SYBR
Polymerase	AmpliTaq DNA Polymerase
Format	Vial
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast or Standard
Label or Dye	SYBR GreenER
Hot Start	Built-In Hot Start
Sufficient For	100 Reactions of 20 μL
Product Line	SYBR

142

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Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Content And Storage	Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq)	2 X
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Product Type	Universal Master Mix II
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
Product Line	TaqMan

143

Applied Biosystems™ TaqMan™ SARS-CoV-2, Flu A/B, RSV RT-PCR Assay Kit

Detect RNA from multiple respiratory viruses simultaneously with the TaqMan SARS-CoV-2, FluA/B, RSV RT-PCR Assay Kit, a highly accurate, real-time PCR multiplex assay that allows you to expand your respiratory sample analysis menu while maintaining low operational costs.

Pricing & Availability

144

Applied Biosystems™ TrueMark™ PGx Comprehensive Plasmid Controls

TrueMark PGx Comprehensive Plasmid Controls contains three linearized multi-target plasmid pools with human genome target sequences commonly used for pharmacogenomic research testing.

Pricing & Availability

145

Promotions Available

Applied Biosystems™ Absolute Q™ 1-step RT-dPCR Master Mix (4X)

Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix (4X) is optimized for use with the QuantStudio Absolute Q Digital PCR System in a simple workflow with minimal processing steps.

Pricing & Availability

146

Invitrogen™ SuperScript™ III Reverse Transcriptase

Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript III
Final Product Type	First-Strand cDNA
Concentration	200 U/μL
Format	Stand-alone Enzyme
Size (Final Product)	Up to 12.3 kb
Reaction Format	Separate components
Reaction Speed	30 to 50 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	50°C
Technique	Reverse Transcription
Starting Material	RNA

147

Applied Biosystems™ SYBR™ Select Master Mix for CFX

A cost-effective solution for real-time PCR applications.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Purity or Quality Grade	UP (Ultra Pure)
Form	Liquid
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Detection Method	SYBR
Polymerase	AmpliTaq DNA Polymerase
Format	Vial
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	AB 7900HT, Standard Mode, AB StepOne™, Fast Mode, AB 7500, Standard Mode, AB 7500, Fast Mode, Bio-Rad CFX96 Touch™, AB StepOnePlus™, Fast Mode, AB 7900HT, Fast Mode, AB StepOne™, Standard Mode, Bio-Rad CFX Connect™, AB 7900 HT 384-Well System, Bio-Rad CFX384 Touch™, AB StepOnePlus™, Standard Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast or Standard
Label or Dye	SYBR GreenER
Hot Start	Built-In Hot Start
Product Line	SYBR

148

Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript III
Final Product Type	First-Strand cDNA
For Use With (Application)	Real Time PCR (qPCR)
Detection Method	qPCR
Format	Kit
Size (Final Product)	Up to 10 kb
Reaction Format	Master Mix
Reaction Speed	60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	42°C
Technique	Reverse Transcription
Starting Material	RNA

149

Invitrogen™ Platinum™ GenoType Tsp DNA Polymerase

Platinum GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications.

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
Polymerase	Tsp Genotyping Polymerase
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Size (Final Product)	500 bp or less
Reaction Format	Separate Components
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Overhang	Blunt
For Use With (Application)	Hot-start PCR

150

Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit

The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and qPCR.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Format	Kit
Reverse Transcriptase	SuperScript IV
Reaction Format	Premixed Components
Final Product Type	First-Strand cDNA
Reaction Speed	10 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	50°C
Technique	Reverse Transcription
Starting Material	Cells
For Use With (Application)	RT-PCR,Real Time PCR (qPCR)

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PCR Supplies

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Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix

Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit