PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.

136 – 150 of 3,335 results

136

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Applied Biosystems™ Fast SYBR™ Green Master Mix

Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Detection Method	SYBR
Polymerase	AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast
Label or Dye	SYBR Green
Product Line	SYBR™

137

Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Format	Kit
Reverse Transcriptase	MultiScribe
Size (Final Product)	Up to 7 kb
Reaction Format	Premixed Components
Final Product Type	First-Strand cDNA
Reaction Speed	30 to 60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Optimal Reaction Temperature	37°C
Technique	Reverse Transcription
Starting Material	RNA
For Use With (Application)	Real Time PCR (qPCR),RT-PCR

138

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0

Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

Pricing & Availability
Specifications

Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,Pathogen Detection,Virus Detection
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Format	Bottle
Product Type	qPCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Sample Type	Environmental Sample, Food
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Product Line	TaqMan™

139

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Applied Biosystems™ TaqMan™ Rodent GAPDH Control Reagents

TaqMan™ Rodent GAPDH Control Reagents

Pricing & Availability

140

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Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/TAMRA™ probe, primer limited)

Eukaryotic 18S rRNA Endogenous Control (VIC™/TAMRA™ probe, primer limited)

Pricing & Availability

141

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Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents

TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.

Pricing & Availability
Specifications

GC-Rich PCR Performance	Low
Format	Tube(s)
Internal Probe Modification	TAMRA™ Quencher (3'), VIC™ (5')
Product Type	Exogenous Internal Positive Control Reagent
Reaction Speed	Standard
Green Features	Sustainable packaging
PCR Method	qPCR
Product Line	TaqMan™

142

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Applied Biosystems™ Human PPIA (Cyclophilin A) Endogenous Control (VIC™/MGB probe, primer limited)

Human PPIA (Cyclophilin A) Endogenous Control (VIC™/MGB probe, primer limited)

Pricing & Availability

143

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit

The Ambion Single Cell-to-C_T™ Kit contains a complete validated workflow for gene expression analysis for samples containing 1–10 cells.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Sample Type	Mammalian Cells
Reaction Speed	7 min.
Purification Time	7 min.
Green Features	Fewer resources used and less waste, less hazardous
Starting Material	1-10 Cells
For Use With (Application)	RT-PCR
PCR Method	2-step RT-qPCR
Product Line	Ambion™, Cells-to-CT™
Detection Method	TaqMan

144

Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix

The SuperScript III First-Strand Synthesis SuperMix is an optimized formulation for first-strand cDNA synthesis from purified poly(A)⁺ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system.

Pricing & Availability

145

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Invitrogen™ SuperScript™ IV Reverse Transcriptase

SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript IV
Final Product Type	First-Strand cDNA
Concentration	200 U/μL
Format	Stand-alone Enzyme
Size (Final Product)	Up to 12.3 kb
Reaction Format	Separate components
Reaction Speed	10 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	50°C
Technique	Reverse Transcription
Starting Material	RNA

146

Invitrogen™ SuperScript™ III Reverse Transcriptase

Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript III
Final Product Type	First-Strand cDNA
Concentration	200 U/μL
Format	Stand-alone Enzyme
Size (Final Product)	Up to 12.3 kb
Reaction Format	Separate components
Reaction Speed	30 to 50 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	50°C
Technique	Reverse Transcription
Starting Material	RNA

147

Thermo Scientific™ dUTP Solution (100 mM)

Thermo Scientific dUTP (2'-deoxyuridine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

Pricing & Availability

148

Thermo Scientific™ Aminoallyl-dUTP Solution (50 mM)

Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.

Pricing & Availability

149

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Format	Tube
Product Type	1-Step qPCR kit
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type	Mammalian Cells
Reaction Speed	7 min.
Reagent Type	Cell Lysis
Green Features	Fewer resources used and less waste, less hazardous
Starting Material	10-100,000 Cells
For Use With (Application)	Gene Expression
PCR Method	1-step RT-qPCR
Product Line	Cells-to-CT™, Power SYBR™ Green
Detection Method	SYBR

150

Invitrogen™ Collibri™ Library Quantification Kit

Invitrogen™ Collibri™ Library Quantification Kit includes a ready-to-use master mix optimized for Illumina NGS library quantification and a library dilution buffer

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Platinum™ II Taq Hot Start DNA Polymerase
GC-Rich PCR Performance	Low
Content And Storage	Store at -20°C.
Format	Kit
Product Type	Library Quantification Kit
For Use With (Equipment)	7500 Fast System, 7900HT Fast System, QuantStudio™, ViiA™ 7 System, StepOne™ System, StepOnePlus™ System
Sample Type	DNA,RNA
PCR Method	qPCR
For Use With (Application)	Illumina NGS Library Quantification
Test Time	Standard
Product Line	Collibri™
Detection Method	SYBR

PCR Supplies

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Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/TAMRA™ probe, primer limited) Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Applied Biosystems™ Human PPIA (Cyclophilin A) Endogenous Control (VIC™/MGB probe, primer limited) Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/TAMRA™ probe, primer limited)

Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents

Applied Biosystems™ Human PPIA (Cyclophilin A) Endogenous Control (VIC™/MGB probe, primer limited)

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit